Acquirement of transgenic rose plants from embryogenic calluses via Agrobacterium tumefaciens
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چکیده
منابع مشابه
Isolation of Agrobacterium Tumefaciens Strains from Crown Gall Disease on Imported Roses Plants in Qazvin Province
Cultivar of roses (Rosa spp.) has been grown in greenhouses in Qazvin region of Iran for local markets. Agrobacterium tumefaciens strains were isolated and identified from six different samples of roses plants imported from the Netherland to Iran. During August and September of 2012, nearly 2-5% of rose plants in two different greenhouses in the province of Qazvin were observed with crown gall ...
متن کاملAn improved procedure for production of white spruce (Picea glauca) transgenic plants using Agrobacterium tumefaciens.
An efficient and reproducible procedure for the transformation of white spruce (Picea glauca [Moench] Voss) embryogenic tissues was developed using A. tumefaciens-mediated gene transfer. Rapidly dividing white spruce embryogenic tissues were co-cultivated with disarmed A. tumefaciens strains containing additional copies of the virulence regions from plasmid PToK47. The plasmid pBi121, containin...
متن کاملDevelopment of Transgenic Tall Fescue Plants from Mature Seed-derived Callus via Agrobacterium-mediated Transformation
We have achieved efficient transformation system for forage-type tall fescue plants by Agrobacterium tumefaciens. Mature seed-derived embryogenic calli were infected and co-cultivated with each of three A. tumefaciens strains, all of which harbored a standard binary vector pIG121Hm encoding the neomycin phosphotransferase II (NPTII), hygromycin phosphotransferase (HPT) and intron-containing β-g...
متن کاملisolation of agrobacterium tumefaciens strains from crown gall disease on imported roses plants in qazvin province
cultivar of roses (rosa spp.) has been grown in greenhouses in qazvin region of iran for local markets. agrobacterium tumefaciens strains were isolated and identified from six different samples of roses plants imported from the netherland to iran. during august and september of 2012, nearly 2-5% of rose plants in two different greenhouses in the province of qazvin were observed with crown gall ...
متن کاملPersistence of Agrobacterium tumefaciens in transformed conifers.
Previous studies have shown that the widely used plant transformation vector Agrobacterium tumefaciens can persist in genetically engineered plants in vitro and in transgenic greenhouse-grown plants, despite the use of counter-selective antibiotics. However, little is known regarding Agrobacterium persistence in tree species. To understand the kinetics of A. tumefaciens decline and persistence ...
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ژورنال
عنوان ژورنال: Journal of Plant Biotechnology
سال: 2010
ISSN: 1229-2818
DOI: 10.5010/jpb.2010.37.4.511